Center for Biofilm Engineering
Abstract:
"Gene Expression and Protein Levels of the Stationary Phase Sigma
Factors, RpoS, in Continuously-Fed Pseudomonas aeruginosa Biofilms"
01-018 Bacteria growing in biofilms experience gradients of
environmental conditions, including varying levels of nutrients and
oxygen. Therefore, bacteria within biofilms may enter distinct
physiological states, depending on the surrounding conditions. In this
study, rpoS expression and RpoS levels were measured as indicators of
stationary phase growth within thick continuously-fed Pseudomonas aeruginosa
biofilms. The level of rpoS expression in a 3-day-old biofilm was
found to be three-fold higher than the average expression in stationary phase
planktonic cultures. In planktonic cultures, oxygen limitation did not
lead to increased levels of RpoS, suggesting that oxygen limitation was not the
environmental signal causing increased expression of rpoS. These
results suggest that bacteria within P. aeruginosa biofilms may exhibit
stationary phase characteristics even when cultured in flow conditions that
continually replenish nutrients.
Xu, K.D., M.J. Franklin, C-H Park, G.A. McFeters, and P.S. Stewart,
"Gene Expression and Protein Levels of the Stationary Phase Sigma Factors,
RpoS, in Continuously-Fed Pseudomonas aeruginosa Biofilms," FEMS
Microb Lett., 199:67-71 (2001).
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