Center for Biofilm Engineering
Abstract:
"Apparent Surface Associated Lag Time in Growth of Primary Biofilm Cells"
00-028 The ability of microorganisms to form
biofilms has been well documented. Bacterial cells make a transition from a
planktonic state to a sessile state, replicate, and subsequently populate a
surface. In this study, organisms that initially colonize a “clean” surface
are referred to as “primary” biofilm cells. The progeny of the first
generation of sessile cells are known as “secondary” biofilm cells. This
study examined the growth of planktonic, primary, and secondary biofilm cells of
a green fluorescent protein producing (GFP+) Pseudomonas aeruginosa PA01.
Biofilm experiments were performed in a parallel plate flow cell reactor with a
glass substratum. Individual cells were tracked over time using a confocal
scanning laser microscope (CSLM). Primary cells experience a lag in their growth
that may be attributed to adapting to a sessile environment or undergoing a
phenotypic change. This is referred to as a surface associated lag time.
Planktonic and secondary biofilm cells both grew at a faster rate than the
primary biofilm cells under the same nutrient conditions.
Correspondence to: A.K. Camper — Email: anne_c@erc.montana.edu
Rice, A.R., M.A. Hamilton, and A.K. Camper, "Apparent Surface Associated
Lag Time in Growth of Primary Biofilm Cells," 40(1):8-15 (2000).
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