Center for Biofilm Engineering
Abstract:
"Activity and Stability of a Recombinant Plasmid-Borne TCE Degradative Pathway in Suspended Cultures"
98-010 The retention and expression of the plasmidborne, TCE degradative toluene-ortho-monooxygenase
(TOM) pathway in suspended continuous cultures of transconjugant Burkholderia cepacia
17616 (TOM31c) were studied. Acetate growth and TCE degradation kinetics
for the transconjugant host are described and utilized in a plasmid loss model.
Plasmid maintenance did not have significant effect on the growth rate of the
transconjugant. Both plasmid-bearing and plasmid-free strains followed Andrews
inhibition growth kinetics when grown on acetate and had maximum growth rates of 0.22 h-1.
The transconjugant was capable of degrading TCE at a maximum rate of 9.7 nmol
TCE/min . mg protein, which is comparable to the rates found for the original
plasmid host, Burkholderia cepacia PR131 (TOM31c).
The specific activity of the TOM pathway was found to be a linear function of growth
rate. Plasmid maintenance was studied at three different growth rates:
0.17/h, 0.1/h, and 0.065/h. Plasmid maintenance was found to be a function of growth
rate, with the probability of loss ranging from 0.27 at a growth rate of 0.065/h to 0.034
at a growth rate of 0.17/h.
Sharp, R.R., J.D. Bryers, W.G. Jones, and M.S. Schields, "Activity and Stability
of a Recombinant Plasmid-Borne TCE Degradative Pathway in Suspended Cultures,"
Biotechnol. Bioengrg. 57(3):287-296 (1998).
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