CBE News Update

Stains for this workshop were donated by Molecular Probes™ - Invitrogen.

A huge thanks to Molecular Probes™ - Invitrogen for giving us this opportunity to learn more about various stains and what they can do for our research imaging.

Diane Gray (right), our colleague at Molecular Probes™, brought 11 fluorescent stains for us to use during this workshop. Many of these stains were either unfamiliar to us or had not been used extensively on biofilms. This made the workshop an experiment for everyone, including CBE staff. Everyone felt free to contribute their ideas and expertise and for some it was a fun "return to the bench top". The resulting images (above) were fascinating and inspirational.

For more information about Molecular Probes™ - Invitrogen detection technologies contact:

Diane Gray
Ph: 541 465-8300 ext 59754
Fax: 541 335-0177

Email: Diane Gray

Diane Gray, Microbiologist

Simply through her enthusiasm for learning and teaching, CBE Betsey Pitts (far left) encourages an atmosphere of open discussion and sharing of experience among participants.

No more sitting on stools...it's time for the "hands on" stuff!. After shrugging on lab coats and finding some goggles and gloves, participants watch CBE Diane Walker (purple gloves) demonstrate biofilm growth reactor sampling.

Now it's your turn. John Novak and Ali Kilic (American Air Liquide) teamed up to prepare their experiment.

Diane Gray, Molecular Probes, was right in there, too. On her right is lab partner, Chia Hung, Sequoia Sciences, Inc.

Victoria Hitchens, FDA and Ron Najafi, NovaCal Pharmaceuticals, Inc. prepare serial dilutions of biofilm sampled earlier.

After lunch, the stained biofilms were observed in situ using epifluorescence microscopy. CBE Willy Davison and Katy McClary, W.L. Gore & Associates discuss the results.

Richard Fiser, Tyco Healthcare (left) and CBE Suriani (Sue) Abdul Rani scan through layers of stained biofilm using the confocal microscope.

THANK YOU TO..... all the participants whose interest and participation made this such a great workshop. CBE Staff: Kelli Buckingham-Meyer Linda Loetterle Betsey Pitts Diane Walker Alessandra Agostinho CBE Students: Suriani Abdul Rani Ellen Swogger Willy Davison Audrey Corbin and a special thanks to Diane Gray, Molecular Probes™ - Invitrogen, for providing the stains and making this workshop such a success. Next Biofilm Methods Workshop June 19, 2006 For information on upcoming workshops, see our Workshops page.	The Fluorescent Stains 1. BacLight™ Green bacterial stain (B35000) BacLight™ Red bacterial stain (B35001) 2. LIVE/DEAD® BacLight™ Bacterial Viability Kit (L7012) 3. BacLight™ Bacterial Membrane Potential Kit (B34950) 4. BacLight™ RedoxSensor™ Green Vitality Kit (B34954) 5. BODIPY® 581/591 C11 (D3861) 6. concanavalin A, Alexa Fluor® 488 conjugate (C11252) 7. concanavalin A, succinylated, Alexa Fluor® 488 conjugate (C21401) 8. Calcofluor™ White M2R (Component B from LIVE/DEAD® Yeast Viability Kit) (L7009) 9. 5-carboxyfluorescein diacetate, acetoxymethyl ester (5-CFDA, AM) (C1345, F34953) 10. BacLight™ RedoxSensor™ CTC Vitality Kit (B34956) 11. FM® 1-43 (T35356)

March, 2006

Volume 9, Issue 3

Biofilm Methods Workshop

February 1, 2006

THANK YOU TO.....

Next Biofilm Methods Workshop

The Fluorescent Stains