A PhD-course (3 units)
Held at BioCentrum, DTU, Copenhagen, Denmark, April 2-6, 2002.
Organizers: Michael Givskov, Søren Molin & Bill Costerton
Number of participants 16
Program
Tuesday, April 2
9-12: Workshop.
Speaker: Bill Costerton CBE : Biofilms as structured communities
Speaker: Søren Molin, BiC: Molecular tools in studies of microbial biofilms
12-14: Lunch, informal interactions with speakers.
14-18: Demonstration exercises 1 & 2 in the lab
Wednesday, April 3
9-12: Workshop.
Speaker. Niels Høiby UC: Chronic Pseudomonas aeruginosa lung infection in
cystic fibrosis - a biofilm disease
Speaker: Claus Moser UC: Rat model of chronic Pseudomonas aeruginosa lung infection
12-14: Lunch, informal interactions with speakers.
14-18: Demonstration exercises 1 & 2 in the lab
Thursday, April 4
9-12: Workshop.
Speaker: Michael Givskov, BiC: Molecular tools for the study of quorum sensing and QS inhibition.
Speaker: Michael Kuhl, Helsinore : Direct examination of biofilm micro-niches
12-14: Lunch, informal interactions with speakers.
14-18: Demonstration exercises 3 & 4 in the lab
Friday, April 5
9-12: Workshop.
Speaker: Paul Stoodley CBE : viscoelastic properties of biofilms
Speaker: Jeff Leid CBE : interactions of phagocytes with biofilms
Speaker: Matt Parsek Northwestern: Biofilms and the study of EPS
12-14: Lunch, informal interactions with speakers.
14-18: Demonstration exercises 3 & 4 in the lab
Saturday, April 6
9-12: Workshop.
Speaker: Tim Tolker Nielsen BiC: Development and dynamics of biofilms
Speaker: Dave Davies CBE : The biofilm phenotype
Speaker: Paul Sturman CBE : On line measurement of biofilm formation
Departure
The laboratory demonstration exercises
1. Structure/function analysis of P. aeruginosa biofilms in relation to quorum sensing activity and physiology. Display of AHL monitor systems in flow-chamber biofilms. Analysis of wild type and mutant strains including quorum sensing inhibition. Growth rate monitors and quantitative image analysis. Supervisor: Morten Hentzer
2. Flow cytometric analysis and cell sorting of biofilm-associated bacteria according to their physiological activity. FACS investigations and preparations of biofilm cells. Correlation between physiological activity and cell size. Isolation of differentially tagged cells. Investigations of live-dead stained cells from antibiotic treated biofilm. Supervisor: Claus Sternberg
3. Viscoelastic properties of biofilms. Supervisor: Paul Stoodley. Demonstration of the interaction of phagocytes with biofilms. Supervisor : Jeff Leid
4. Demonstration of proteomic analysis. Supervisor : Dave Davies. Microelectrodes for in situ measurements in biofilms. Supervisor: Michael Kuhl
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| Workshop Agendas
Medical Biofilms (Tokyo, Japan)
Chronic Infections (Rome, Italy)
Subsurface Biofilm, (Frankfurt, Germany)
Molecular Tools in Biofilms (Concepcion, Chile)
Biofilm Workshop (Copenhagen, Denmark and Sydney, Australia)
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