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Center for Biofilm Engineering
Movie Description:
Seeding |
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| A Pseudomonas aeruginosa PAO1 biofilm
microcolony which had differentiated into two distinct cell
phenotypes; highly motile cells in the interior of the clusters (long
white arrow) and cells which remained stationary making an outer
cluster "wall' (small black and white arrows). The differentiation of
the microcolony is suggestive of a primative fruiting body in which
dipersing "seeding" cells are released to colonize new niches. The
scale bar is 20
µm.
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| Movie Authors: Purevdorj-Gage,
B. and Stoodley, P. |
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| Reference:
Purevdorj-Gage, B., Costerton, J.W. and Stoodley, P. 2005.
Phenotypic differentiation and seeding dispersal in non-mucoid and
mucoid Pseudomonas aeruginosa biofilms. Microbiology
(151):1569-1576
Further Reading:
Purevdorj, B. and Stoodley, P. 2004. Hydrodynamic considerations of
biofilm structure and behavior. In Microbial Biofilms. Ghannoum,
M.A. and O'Toole, G. (Eds). ASM Press, Washington DC. pp 160-173.
Hall-Stoodley, L., Costerton, J.W., and Stoodley, P. 2004. Bacterial
biofilms: From the environment to infectious disease. Nature
Reviews Microbiology. 2(2): 95-108.
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Movie Description:
Dispersal
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| Time-lapse movie showing the seeding dispersal process
in which cells evacuate the clusters from local 'breakout' points in
the cell cluster 'walls'. The location of the breakout points appeared
to be random and independent of the flow direction. The movement of
bacterial cells out of the cell cluster and across the surface of the
glass flow cell was very similar to the swarming motility observed on
solid agar surfaces. Bulk flow was from left to right at an average
velocity of 1.7 cm/s. The white arrows indicate the dispersing cells.
The scale bar is 20 µm. |
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| Movie Authors: Purevdorj-Gage,
B. and Stoodley, P.
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|
| Reference:
Purevdorj-Gage, B., Costerton, J.W. and Stoodley, P. 2005.
Phenotypic differentiation and seeding dispersal in non-mucoid and
mucoid Pseudomonas aeruginosa biofilms. Microbiology
(151):1569-1576
Further Reading:
Purevdorj, B. and Stoodley, P. 2004. Hydrodynamic considerations of
biofilm structure and behavior. In Microbial Biofilms. Ghannoum,
M.A. and O'Toole, G. (Eds). ASM Press, Washington DC. pp 160-173.
Hall-Stoodley, L., Costerton, J.W., and Stoodley, P. 2004. Bacterial
biofilms: From the environment to infectious disease. Nature
Reviews Microbiology. 2(2): 95-108.
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