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Center for Biofilm Engineering
Movie Description:
Nutrient starved detachment
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| Example animation from a single
experiment using the BacLAB computer model. A 512µm x512µm surface was
randomly inoculated with 28 (potentially overlapping) clusters of
bacteria. Biofilm growth and detachment were simulated for 500 hours
in the absence of bulk fluid shear. This animation demonstrates the
degree of structural heterogeneity, and the dynamic nature of the
structure, attained when detachment is regulated by nutrient
starvation.
Movie Authors: Hunt, S.M.,
Hamilton, M.A. and Stewart, P.S.
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| Reference:
"Hypothesis for the Role of Nutrient Starvation in Biofilm
Detachment"
Hunt, S.M., Werner, E.M., Huang, B., Hamilton, M.A. and Stewart,
P.S.
Appl. Environ. Microbiol., 70(12):7418-7425 (2004)
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Seething
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| This is a Pseudomonas aeruginosa biofilm cell
cluster observed by transmission mode time lapse confocal scanning
laser microscopy. In the interior of the cell cluster, a few
motile cells exhibit rapid swirling motion. Cells in the outer
shell of the biofilm cluster are immobile. Dissolution of the
central region of the cluster may be a prelude to detachment and
dispersal of biofilm cells.
Movie Authors: Werner, E.M. and Stewart, P.S.
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| Reference:
"Hypothesis for the Role of Nutrient Starvation in Biofilm
Detachment"
Hunt, S.M., Werner, E.M., Huang, B., Hamilton, M.A. and Stewart,
P.S.
Appl. Environ. Microbiol., 70(12):7418-7425 (2004)
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