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Center for Biofilm Engineering
Movie Description:
Movies showing the effect of powered toothbrushing by Philips Sonicare®
toothbrush on a Streptococcus mutans biofilm
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Fluid motion removes biofilm
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| Movie showing the removal of
a Strep. mutans biofilm by powered brushing for 15s with a
Philips Sonicare® Advance toothbrush. Individual microcolonies in the
biofilm are seen as white patches. The biofilm was grown on a glass
microscope slide in a drip flow reactor. The toothbrush bristles were
positioned 0.5 mm above the biofilm and, even without direct contact,
the brush removed approximately 70% of the biofilm through the fluid
motion created by the rapid bristle oscillation. Click image or link
below to play movies.
Play Movie
02-M010
Movie Authors: Heersink, J., J.W. Costerton and P.
Stoodley
Supplemental Online Material:
Heersink, J., Costerton, W.J. and Stoodley, P. 2003.
Influence of the
Sonicare® toothbrush on the structure and thickness of laboratory
grown Streptococcus mutans biofilms assessed by digital time-lapse and
confocal microscopy. American Journal of Dentistry. 16(2): 79-83.
Further Reading:
H. Adams, M. Winston, J. Heersink, K. Buckingham-Meyer, W.J.
Costerton, and P. Stoodley. 2002.
Development of a
laboratory model to assess the removal of biofilm from interproximal
spaces by powered tooth brushing. American Journal of Dentistry.
15(Special issue):12B-17B. |
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Bristle contact power
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| Low power movie
showing the Philips Sonicare® Advance toothbrush cutting a swath
through a Strep. mutans biofilm. The brush moved from top right
down. The slide is then positioned so the unbrushed side is on the
left and the brushed side on the right. When the bristles were in
contact the brush removed over 99.9% of the biofilm. Click the picture
to see the movie. A 3D confocal movie showing the biofilm in the
region of the black arrow is included in the next frame. |
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| Play
Movie
02-M011
Movie Authors: Heersink, J., J.W. Costerton and P.
Stoodley
Supplemental Online Material:
Heersink, J., Costerton, W.J. and Stoodley, P. 2003.
Influence of the
Sonicare® toothbrush on the structure and thickness of laboratory
grown Streptococcus mutans biofilms assessed by digital time-lapse and
confocal microscopy. American Journal of Dentistry. 16(2): 79-83.
Further Reading:
H. Adams, M. Winston, J. Heersink, K. Buckingham-Meyer, W.J.
Costerton, and P. Stoodley. 2002.
Development of a
laboratory model to assess the removal of biofilm from interproximal
spaces by powered tooth brushing. American Journal of Dentistry.
15(Special issue):12B-17B. |
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Powered brushing
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| 3D confocal
movie of the Strep. mutans biofilm shown in the in the above
frame. The bright dots are not individual cells but clusters of cells
in the biofilms. The biofilm was stained with Molecular Probes
Live/Dead BacLight kit so that live cells appeared green and dead
cells, red. Click picture to see the movie showing the 3D effect.
Play Movie
02-M012
Movie Authors: Heersink, J. and P. Stoodley
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| Supplemental
Online Material:
Heersink, J., Costerton, W.J. and Stoodley, P. 2003.
Influence of the
Sonicare® toothbrush on the structure and thickness of laboratory
grown Streptococcus mutans biofilms assessed by digital time-lapse and
confocal microscopy. American Journal of Dentistry. 16(2): 79-83.
Further Reading:
H. Adams, M. Winston, J. Heersink, K. Buckingham-Meyer, W.J.
Costerton, and P. Stoodley. 2002.
Development of a
laboratory model to assess the removal of biofilm from interproximal
spaces by powered tooth brushing. American Journal of Dentistry.
15(Special issue):12B-17B.
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Disruption of biofilm in an interproximal model
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| Movie showing
the displacement of a patch of Strep. mutans biofilm from a
space modeling the interproximal space between teeth by powered
brushing with a Philips Sonicare Elite® toothbrush. Even though the
bristles were not in direct contact with the biofilm, this research
demonstrated that the fluid motion created by the bristles may remove
dental plaque biofilm from hard to reach places in the mouth.
Play Movie
Use Quick Time version 6. Free download at
www.quicktime.com.
02-M013
Higher resolution
Movie Authors: Adams, H., M. Winston and P. Stoodley |
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| Supplemental Online
Material:
H. Adams, M. Winston, J. Heersink, K. Buckingham-Meyer, W.J.
Costerton, and P. Stoodley. 2002.
Development of a
laboratory model to assess the removal of biofilm from interproximal
spaces by powered tooth brushing. American Journal of Dentistry.
15(Special issue):12B-17B.
Further Reading:
J. Heersink, W.J. Costerton and P. Stoodley. 2003.
Influence of the Sonicare® toothbrush on the structure and thickness of laboratory
grown Streptococcus mutans biofilms assessed by digital
time-lapse and confocal microscopy. American Journal of Dentistry,
16(2): 79-83.
Acknowledgements: This research was funded by Philips
Oral Healthcare. Digital optimization and construction of Quick
Time movies by James Meyer. |
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