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Center for Biofilm Engineering
Movie Description:
Human leukocytes adhere, penetrate, and respond to Staphylococcus
aureus biofilms
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Leukocytes interacting with Staph. aureus biofilm under
fluid shear
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| Human leukocytes flowing
past a 7-day Staph. aureus biofilm growing on the wall of a
glass flow cell. Some of the leukocytes were entrapped in channels in
the biofilm (white arrow). Flow was laminar. Time period = 3 sec.
Video sequence collected by P. Stoodley, S. Wilson, and J.G. Leid
Play Movie
02-M006
Movie Authors: J.G. Leid, M.E. Shirtliff, W.J.
Costerton, P. Stoodley
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| Supplemental
Online Material:
J.G. Leid, M.E. Shirtliff, W.J. Costerton and P. Stoodley.
Human leukocytes adhere
to, penetrate, and respond to Staphylococcus aureus biofilms.
Infection and Immunity. 70(11):6339-6345
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3D movie of PMN embedded in Staph. aureus biofilm grown
under fluid flow
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| This 3D confocal image shows
a single PMN leukocyte embedded in the Staph. aureus biofilm
(green and red) after a 1 hour exposure under fluid shear. Molecular
Probes BacLight was used for staining. The bacterial cells stained
predominantly green indicating that their viability had not been
compromised after the leukocyte challenge. However, the cleared zone
around the leukocyte (L) indicates that the leukocyte may have had a
very localized effect on the biofilm. It was noted that although
initially leukocytes stained green after 1 hour incubation they
appeared red indicating that the membranes had been permeabilised,
presumably by the DMSO present in the staining kit. |
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Confocal image collected by S. Wilson, P. Stoodley, and J.G. Leid
Play Movie
02-M007
Movie Authors: J.G. Leid, M.E. Shirtliff, W.J.
Costerton, P. Stoodley
Supplemental Online Material:
J.G. Leid, M.E. Shirtliff, W.J. Costerton and P. Stoodley.
Human leukocytes adhere
to, penetrate, and respond to Staphylococcus aureus biofilms.
Infection and Immunity. 70(11):6339-6345
|
|
3D movie of human leukocytes embedded in Staph. aureus
biofilm grown under fluid flow
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| This 3D confocal image shows
multiple leukocytes (L) embedded in a 7 day Staph. aureus
biofilm. The leukocytes were often observed aggregated in channels
(Ch) in the biofilm. It is not clear if the leukocytes were
transported into existing channel by the fluid flow or if they
"burrowed" through the biofilm creating the channels as they went. Our
evidence suggests that both mechanisms facilitated the penetration of
leukocytes into the biofilm.
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| Confocal image
collected by S. Wilson, P. Stoodley, and J.G. Leid
Play Movie
02-M008
Movie Authors: J.G. Leid, M.E. Shirtliff, W.J.
Costerton, P. Stoodley
Supplemental Online Material:
J.G. Leid, M.E. Shirtliff, W.J. Costerton and P. Stoodley.
Human leukocytes adhere
to, penetrate, and respond to Staphylococcus aureus biofilms.
Infection and Immunity. 70(11):6339-6345
|
|
3D movie of human leukocytes embedded in Staph. aureus
biofilm grown under static conditions
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This 3D confocal image shows
many leukocytes (L) attached to a Staph. aureus biofilm grown
as a static culture. These biofilms consisted of more extracellular
polymeric slime (EPS) than those grown under flow. The EPS stained red
with the Molecular Probes BacLight. Single green and red bacterial
cells (Sa) were interspersed in the microcolony. The majority of the
bacterial cells were green indicating viability, membrane compromised
cells stained red.
Confocal image collected by P. Stoodley and J.G. Leid
Play Movie
02-M009
Movie Authors: J.G. Leid, M.E. Shirtliff, W.J.
Costerton, P. Stoodley |
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| Supplemental
Online Material:
J.G. Leid, M.E. Shirtliff, W.J. Costerton and P. Stoodley.
Human leukocytes adhere
to, penetrate, and respond to Staphylococcus aureus biofilms.
Infection and Immunity. 70(11):6339-6345 |
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