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Center for Biofilm Engineering
Movie Descriptions:
Influence of hydrodynamics and cell signaling on the structure and
behavior of Pseudomonas aeruginosa biofilms
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Rippling in Pseudomonas aeruginosa PAO1 biofilms
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| Pseudomonas aeruginosa PAO1
biofilm growing in a glass flow cell under turbulent flow. The flow
velocity was 1 m/s. The biofilm formed streamlined cell clusters and
ripple like structures. This time-lapse movie shows that the ripples
moved slowly downstream over a 20 hour period. The motion of biofilm
along surfaces may have implications for the dissemination of
infection or contamination in medical and industrial contexts. The
biofilm was 4 days old. Scale bar = 200 microns.
Supplemental Online Material:
Purevdorj, B., Costerton, J.W., and Stoodley, P. 2002.
Influence of hydrodynamics and cell signaling on the structure and
behavior of Pseudomonas aeruginosa biofilms. Appl. Environ.
Microbiol. 68(9):4457-4464.
Further Reading:
Stoodley, P., Z. Lewandowski, J.D. Boyle and H.M. Lappin-Scott, "The
Formation of Migratory Ripples in a Mixed Species Bacterial Biofilm
Growing in Turbulent Flow," J. Environ. Microbiol. 1(5). 447-455
(1999). |
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Rippling in Pseudomonas aeruginosa JP1 biofilms
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| Biofilm formed by the cell signaling
ΔlasI::tet, lasI null mutant which does not produce the quorum
sensing (QS) signal N-3-oxo-dodecanoyl homoserine lactone (OdDHL). The
5 day old biofilm was grown under turbulent flow (flow velocity = 1
m/s) in a square glass capillary flow cell. The biofilm formed similar
structures to the parental PAO1 WT strain. These data suggest that QS
alone is not necessarily required for biofilm formation and that other
factors such as hydrodynamics can play an equally, if not more
significant, role in determining the biofilm structure. It is likely
that the concentration of signals in biofilms will be strongly
influenced by the flow conditions. In flowing, open, systems it is
possible that cell signals are “washed” out of the biofilm so that the
build up of signal concentrations to QS levels may delayed (or never
achieved) with respect to quiescent systems. Scale bar = 100 microns.
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| Movie Author: B. Purevdorj
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| Supplemental Online Material:
Purevdorj, B., Costerton, J.W., and Stoodley, P. 2002.
Influence of hydrodynamics and cell signaling on the structure and
behavior of Pseudomonas aeruginosa biofilms. Appl. Environ.
Microbiol. 68(9):4457-4464.
Further Reading:
Stoodley, P., Lewandowski, Z., Boyle, J.D. and Lappin-Scott, H.M.
1999. The formation of
migratory ripples in a mixed species bacterial biofilm growing in
turbulent flow. Environ. Microbiol. 1:447-457.
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